Anti-MKLP: rabbit polyclonal

Figure 1.  Western blot analysis of anti-MKLP1 antibody.  Protein samples were separated by electrophoresis and transferred to PVDF membrane as described in the methods.  AKIN06 antibody was diluted to 500 ng/ml (1:500) for Western blot analysis.  MKLP1 is detected in 20 µg of mitotic HeLa cell extract (see arrow). Molecular weight markers are from Invitrogen. 

Figure 2.  Western blot of Kinesin Motor Proteins probed with AKIN06 antibody.  Chemiluminescence detection of 1 ng recombinant MKLP1 (lane 1, arrow head).  Other lanes shown on the blot are recombinant motor domains of CENPE (lane 2), Chromokinesin (lane 3), Eg5 (lane 4),  KIF3C (lane 5), MCAK (lane 6), Kinesin Heavy Chain (lane 7). For all motor proteins besides MKLP1, 100 ng of recombinant protein was used.   MKLP1 was the only motor protein that was detected.

Figure 3. Immunoprecipitation of human MKLP1 from HeLa extracts using AKIN06.  Western blot of HeLa cell lysate (Lane 1), IP supernatant after immunoprecipitation (Lane 2), MKLP1 immunoprecipitated with AKIN06 (98 kDa, see arrow) eluted from Protein A beads (lane 3), and control immunoprecipitation reaction with normal rabbit IgG (lane 4).  Lanes 3 and 4 also show IgG heavy chain (55 kDa, arrow head). The Western blot was probed with 500 ng/ml (1:500 dilution) of AKIN06 antibody.