- Home »
- Tubulin protein: Agaricus bisporus
Tubulin protein: Agaricus bisporus
Product Uses Include
- Screening for fungal tubulin specific drugs
- Examining interactions of proteins with fungal tubulin
Tubulin protein has been isolated from the mushroom, Agaricus bisporus, and purified by anion exchange chromatography. Tubulin consists of one α and one β subunit. Each subunit is 55 kDa in size, as demonstrated by SDS-PAGE analysis. Typically, the molar equivalent of tubulin is defined as the heterodimer, which has a molecular weight of 110 kDa. Tubulin is supplied as a white lyophilized powder in the heterodimeric form.
Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein from 4-20% SDS-PAGE. F001 is determined to be >90% fungal tubulin
The biological activity of F001 is assessed by a tubulin polymerization assay. The polymerization of tubulin into microtubules is detected by measuring the optical density at 340 nm (see Figure 2). Under the experimental conditions defined below, a 100 µl solution of F001 at 1.0 mg/ml in a 1/2 area 96-well plate results in a change in optical density of 0.28-0.30 within 1 h at 30°C.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at email@example.com
Coming soon! For the most recent publications citing this product, please contact our Technical Service department at firstname.lastname@example.org
Coming soon! If you have any questions concerning this product, please contact our Technical Service department at email@example.com
- New Products
- Actin Products
- Activation Assays
- Custom Services
- ECM Proteins
- HTS & Bulk
- Kits & Assays
- Motor Proteins
- Other Proteins
- Small G-proteins
- Tubulin & FtsZ Products
You have no items in your shopping cart.
You have no items to compare.
5/3/13Life Inside a Microtubule Read more
3/31/13Tau PTMs as Therapeutic Targets Read more