Product Uses Include
- In vitro binding and inhibition of prenylated Rho proteins
- RhoGDI protein interaction studies
Material
Rho gunanine nucleotide dissociation inhibitor-1 (RhoGDI) binds with high affinity to Cdc42, Rac and Rho family members that have been post-translationally modified at their carboxy-terminal with a prenyl group and with a much lower affinity to the corresponding non-prenylated proteins. RhoGDI is thought to solubilize the prenylated proteins and regulate their association with cellular membranes. Recombinant RhoGDI is supplied as a lyophilized powder. Reconstitution of the protein in water to 1 mg/ml leaves the protein in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent (Cat. # ADV02).
Recombinant RhoGDI has been expressed as a GST fusion protein in a bacterial system. The recombinant protein is 55 kDa in size, consisting of the RhoGDI protein (27 kDa) and an amino-terminal 28 kDa GST tag.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are 90% pure.
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Figure 1: GST-RhoGDI protein purity determination. A 20 µg sample of GDI01 was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.
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Biological Activity
Biological activity of GST-RhoGDI protein was determined by the ability of the protein to inhibit GDP exchange on geranylgeranylated Cdc42
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
Product Description
MSDS
Datasheet
Cat #
GDP dissociation inhibitor protein: GST tagged: Homo sapiens recombinant
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GDI01
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Yamashita, T. and Tohyama, M. (2003). The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI. Nat. Neurosci. 6, 461-467.
Guo, Z., Liu, L., Cafiso, D. and Castle, D. (2002). Perturbation of a very late step of regulated exocytosis by a secretory carrier membrane protein (SCAMP2)-derived peptide. J. Biol. Chem. 277, 35357-35363.
Mehta, D., Rahman, A. and Malik, A. B. (2001). Protein kinase C-α signals rho-guanine nucleotide dissociation inhibitor phosphorylation and rho activation and regulates the endothelial cell barrier function. J. Biol. Chem. 276, 22614-22620.
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