Material
Tubulin and microtubule associated proteins (MAPs) has been purified from porcine brain by an adaptation of the method of Shelanski et al. (1). Tubulin is supplied as a white lyophilized powder. The protein composition is approximately 70% tubulin (55 kDa heterodimer) and 30% MAPs. The MAPs in this product act to stabilize microtubules and to enhance tubulin polymerization. MAP rich tubulin can polymerize efficiently at 1-2 mg/ml.
MAP-rich tubulin is also available from a bovine source (Cat. # ML113).
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are approximately 70% tubulin and 30% MAPs.
Biological Activity
One unit of MAP-rich tubulin is defined as 5.0 mg of protein (as determined by the Precision Red Protein Assay Reagent, Cat. # ADV02). MAP-rich tubulin will polymerize efficiently at protein concentrations above 1mg/ml and temperatures above 24°C.
References
- Shelanski M. L., et al. (1973). Proc. Natl. Acad. Sci. USA. 70, 765-768
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Product Description
MSDS
Datasheet
Cat #
Tubulin protein: MAP rich: porcine brain
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ML116
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