The Acti-stain™ line of fluorescent phalloidins has been developed with an emphasis on creating exceptionally bright and stable probes at an economical price. Side-by-side comparisons with leading competing products demonstrate that you will enjoy savings while not sacrificing the quality of the stain when switching to an Acti-stain™ probe. Additionally, these probes are compatible with popular filter sets such as FITC, TRITC and Cy5. The combination of in-house manufacturing, stringent quality control and convenient packaging provides a great value. Give them a try and see for yourself.
Product Uses Include
Stain F-actin in fixed cells
Stabilize actin filaments in vitro
Visualize actin filaments in vitro
Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures.
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Cytoskeleton's Acti-stain products have been cited many times since their introduction. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.
Andersen et al., 2012. Escherichia coli Uropathogenesis In Vitro: Invasion, Cellular Escape, and Secondary Infection Analyzed in a Human Bladder Cell Infection Model. Infect. Immun. v 80, pp 1858-1867.
Question 2: What fixation conditions are recommended when staining with phalloidin conjugates?
Answer 2: Fluorescent phalloidins only bind to the native quaternary structure of F-actin which provides a low background. The correct fixation condition for phalloidin binding is 3.7% (v/v) paraformaldehyde in PBS for 10 min because it retains the quaternary protein structure which is necessary for high affinity binding of phalloidin. Methanol fixation destroys the native conformation and hence is not suitable for F-actin staining with phalloidin.
For more information about staining procedures and specifications of the Acti-stain series, please click here.