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Anti-cofilin: rabbit polyclonal

Anti-cofilin: rabbit polyclonal

*Amount

1x50 ug (Cat. # ACFL02-A) +$245.00
3x50 ug (Cat. # ACFL02-B) +$595.00

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Product Uses
This antibody is recommended for detection of cofilin in human, mouse, rat, and Xenopus (Fig. 2). The following protocols have been tested with this antibody:

	Western Blot	Immunocytochemistry	ELISA	Immunoprecipitation
	Yes	Yes	Yes	No

Material
ACFL02 is provided as an affinity purified rabbit polyclonal antibody. Cofilin is an actin binding protein that regulates actin dynamics and reorganization through monomer binding and severing of F-actin (reviewed in Bamburg. 1999. Annu Rev Cell Dev Biol. 14:185-230). The antibody was raised against the N-terminal 13-22 amino acids of human cofilin1. Native cofilin has a predicted molecular weight of 19 kD and migrates on an SDS-PAGE gel at 21 kD (Yonezawa et al., 1987; Cell Structure and Function 12:443-452). Platelet cell extract (1 mg; Cat. # EXT01) is included as a Western blot positive control (see Fig. 1). ACFL02 is supplied as a lyophilized white powder.

	Figure 1. Western blot analysis of anti-cofilin antibody (Cat. # ACFL02). Protein samples were separated by electrophoresis and transferred to PVDF membrane as described in the methods. ACFL02 antibody was diluted to 500 ng/ml (1:500) for Western blot analysis. Cofilin was detected in 20 µg of platelet cell extract (see arrow). Molecular weight markers are from Invitrogen.
	Figure 2. Western blot of cell extracts probed with anti-cofilin antibody (Cat. # ACFL02). Chemiluminescence detection of cofilin (see arrow) in 50 µg human HeLa cell extract (lane 5). Other lanes shown on the blot are Drosophila S2 (lane 1), Xenopus A6 (lane 2), mouse Swiss 3T3 (lane 3), rat NRK (lane 4), and human platelet (lane 6) cell extracts (50 µg each). Molecular weight markers are from Invitrogen.
	Figure 3. Immunofluorescence images of mouse Swiss 3T3 cells stained with anti-cofilin antibody (Cat. # ACFL02). Mouse Swiss 3T3 cells were grown to semi-confluency and fixed with 3.7% formaldehyde. Immunofluorescence staining using 500 ng/ml (1:250 dilution) ACFL02 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of goat anti-rabbit rhodamine conjugated antibody (Cat. # RG05). DNA (blue) was stained with 100 nM DAPI in PBS. Photograph was taken with a 20X objective lens.

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Anti-cofilin: rabbit polyclonal

Anti-cofilin: rabbit polyclonal

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