Product Uses Include
• Resuspension of actin protein
• Dilution and storage of G-actin protein
Material
This buffer contains 5 mM Tris-HCl pH 8.0 and 0.2 mM CaCl2. Used as a general G-actin (monomer) buffer with the addition of 0.2 mM ATP (see Cat. # BSA04) and 0.5 mM DTT (A-buffer). The buffer can be changed to a general F-actin (filament supporting) buffer by the addition of 1/10th volume of actin polymerization buffer (see Cat. # BSA02).
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
Product Description
MSDS
Datasheet
Cat #
General actin buffer: 1x stock
|
|
|
BSA01
|
|
Coming soon! For the most recent publications citing this product, please contact our Technical Service department at tservice@cytoskeleton.com
Question 1: Can the general actin buffer be stored with ATP added or should it be added fresh?
Answer 1: On the day of the experiment, the general actin buffer (Cat. # BSA01) should be supplemented with 0.2 mM ATP (Cat. # BSA04) to create an optimal actin monomer buffer. We do not recommend storing the actin buffer with ATP in it. Prepare the actin buffer + ATP in a volume that is needed for the experiment. Calcium ions and ATP are required for actin conformation. In addition, ATP is hydrolysed during actin polymerization and is required for the polymerization process.
Question 2: What is the composition of Cytoskeleton’s general actin buffer?
Answer 2: Upon resuspension in 100 ml of sterile de-ionized water, the buffer (Cat. # BSA01) will be at 1X strength buffer and contain: 0.2 mM calcium chloride and 5 mM Tris-HCl, pH 8.0.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
BSA04
Cat. # PHDR1
BST06
