Product Uses Include
- Identification and characterization of smooth muscle actin binding proteins
- In vitro smooth muscle actin polymerization studies
Material:
Smooth muscle actin has been purified from chicken gizzard. The isotype composition of chicken gizzard smooth muscle actin is approximately 80% γ smooth muscle and 20% β cytoplasmic actin (1). Chicken gizzard smooth muscle actin has an approximate molecular weight of 43 kDa and is provided as a lyophilized white powder.
Purity:
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. Smooth muscle actin is >99% pure (see Figure 1).
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Figure 1. Smooth Muscle Actin Protein Purity Determination. A 100 µg sample of chicken gizzard smooth muscle actin (molecular weight approx. 43 kDa) was separated by electrophoresis in a 12% SDS-PAGE system, and stained with Coomassie Blue. Protein quantitation was determined with the Precision Red Protein Assay Reagent (Cat. # ADV02). |
Biological Activity:
The biological activity of smooth muscle actin is determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the smooth muscle actin polymerizes in this assay.
References
- North, A. J. et al. 1994. J. Cell Sci. 107, 445-455
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
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For a guide to performing actin polymerizations with this actin product please click here.
Product Description
MSDS
Datasheet
Cat #
Actin protein (>99% pure): chicken gizzard muscle
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AS99
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