Alpha-skeletal actin protein has been purified from rabbit skeletal muscle and has an approximate molecular weight of 43 kDa. AKL95 is supplied as a white lyophilized powder.
Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. AKL95 is 95–97% pure alpha-skeletal muscle actin. Potential contaminants may include α-actinin (100 kDa), actin dimers or doublets (86 kDa), and CapZ (33 kDa)
The biological activity of muscle actin is assessed by its ability to efficiently polymerize into filaments in vitro, which can be separated from unpolymerized components using a spin-down assay. Rigorous quality control ensures that >80% of the muscle actin polymerizes under these conditions.
Cat. #AKL95
