Cytoskeleton provides a range of antibodies raised to the Rho family of small G-proteins. They have exceptional isoform specificity and have been quality checked by Western blot, immuno-fluorescence in situ, ELISA and immuno-precipitation in the QC Max protocol. For more information on each antibody please click on the pdf icon below to see the datasheet.
In addition, Cytoskeleton has developed a unique line of fluorescent phalloidins with improved brightness and stability compared to other conjugates such as Alexa fluor and Cy dyes, for more information see the Acti-stain information page.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com.
Cytoskeleton's antibodies products have been cited hundreds of times over the past 18 years. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.
Anti-Cdc42: mouse monoclonal (Cat. # ACD03)
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| Giordano Pula and Alastair W. Poole. Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin a2b1. Platelets, 2008, Vol. 19, No. 3, Pages 199-210 , DOI: 10.1080/09537100701777303 |
| Akio Shimizu and Akiko Mammoto et al. ABL2/ARG Tyrosine Kinase Mediates SEMA3F-induced RhoA Inactivation and Cytoskeleton Collapse in Human Glioma Cells. Journal of Biological Chemistry, 283, 27230-27238. DOI: 10.1074/jbc.M804520200 |
Anti-RhoA: mouse monoclonal (Cat. # ARH03)
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| Khan et al. (2011). Geranylgeranyltransferase type I (GGTase-I) deficiency hyperactivates macrophages and induces erosive arthritis in mice. J Clin Invest doi:10.1172/JCI43758. |
Q: How do I know whether the Cdc42 monoclonal antibody is good for Western blotting and IF?
A: The datasheet (Cat. # ACD03) and the website's individual product page (acd03.html) descibe the applications for all Cytoskeleton's antibodies. The Cdc42 antibody (Cat. # ACD03) was raised against a peptide sequence of human Cdc42 (amino acids 123-141). The Cdc42 antibody will not cross-react with a ten-fold excess of RhoA, RhoB, RhoC, Rac1, Rac2 or Rac3 proteins. Non-specific bands have been observed at higher molecular weight in about 50% of cell lines tested. The antibody has been tested by western blotting and immunocytochemistry. Please see the Citations tab for references that have used this antibody.
Q: Can I test a sample of the RhoA monoclonal antibody?
A: All of Cytoskeleton’s antibodies are rigorously quality-controlled and tested under the conditions described in the product datasheet. In the rare event of a problem with an antibody, please contact our technical service group (tservice@cytoskeleton.com) for assistance. Free samples of the RhoA antibody (cat# ARH03) are not available. However, please contact Cytoskeleton’s customer service department (303-322-2254 or cserve@cytoskeleton.com) to check on any special discount offers.
For more information, click on the Document tab above to see the datasheet.
