Acetyl Lysine Antibody Mouse Monoclonal
Western Blot using Acetyl Lysine Antibody
Cytoskeleton's Anti-Acetyl Lysine Antibody Cat. # AAC01 recognizes 0.005 ng of chemically acetylated BSA and is comparable in sensitivity to other commercially available antibodies. Unlike other Anti-Acetyle Lysine antibodies this antibody does not show cross reactivity with non-acetylated BSA. To see the full Western blot comparison, see the Optimized Protocols or the product datasheet.
Immunoprecipitation using Acetyl Lysine Antibody
Ability of AAC01 to IP histones was compared to other commercially available antibodies. Cytoskeleton's Anti-Acetyl Lysine Antibody provides clear advantages for IP applications. Each tube is sufficient for approximately 20 IP assays. To see the full Immunoprecipitation comparison, see the Optimized Protocols or the product datasheet.
Immunofluorescence using Acetyl Lysine Antibody
Human epidermoid carcinoma A431 cells, untreated (top) or treated (bottom) with TSA (5 mM for 12 h), were stained as described in the method. Acetylated cytoplasmic and nuclear proteins were visualized in green fluorescence. Note that in contrast with the untreated control, acetylated microtubule network is clearly visible in TSA-treated sample. The fluorescent nuclear intensities indicate the high abundance of acetylated proteins in the nucleus. To see the full Immunofluorescence protocol, see the Optimized Protocols or the product datasheet.
ChIP using Acetyl Lysine Antibody
Chromatin was prepared from A431 cells, either untreated or TSA-treated (5 mM, 4 hrs). Briefly, cells were fixed with 1% formaldehyde for 10 min and enzymatically-sheared chromatins were immunoprecipitated by using anti-acetyl antibodies (1:100 dilution). The promoter region of housekeeping gene GAPDH was amplified by a primer pair and PCR products were analyzed by 2% agarose gel-electrophoresis. To see the recommended ChIP protocol, see the Optimized Protocols or the product datasheet.
Protein Acetylation Background
Acetylation of proteins can occur as a co-translational or post-translational modification (PTM) (1). Co-translational acetylation occurs at the N-terminal of approximately 85% of mammalian proteins, it is irreversible and is thought to be important in protein stability, localization and synthesis (1). Post-translational acetylation occurs on the epsilon amino group of lysine residues as a reversible and highly dynamic PTM that is known to be a key regulator in multiple cellular events, including chromatin structure, transcription, metabolism, signal transduction and cytoskeletal regulation (2-3). To date over 4,000 proteins have been identified as targets for PTM acetylation which is comparable to phosphorylation in cellular prevelance (3). Antibody AAC01 detects acetyl lysine PTMs.
1 Bogdan P. and Sherman F. 2002. The diversity of acetylated proteins. Genome Biol. 3 (5): reviews 0006.
2 Lundby A. et al. 2012. Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and cellular patterns. Cell Reports 2:419-431.
3 Sadoul K. et al. 2010. The tale of protein lysine acetylation in the cytoplasm. J. Biomed. Biotech. 2011:1-15.
4 Golemis EA et. Al, Protein-Protein Interactions, CSHLP, 2005, p67
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Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
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Certificate of Analysis: Lot 013
LaBarge et al. 2015. p300 is not required for metabolic adaptation to endurance exercise training. The FASEB Journal article doi: 10.1096/fj.15-281741.
This paper uses anti-acetyl lysine to probe extracts of transgenic mouse muscle tissue, looking for the effects of a knocked out HDAC gene.
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