• In vitro motility studies with non-muscle myosins
• In vitro modeling of the cytoskeleton in the cell cortex
• Study in vivo actin dynamics by labeling of free barbed ends of actin filaments
• Study actin-binding proteins (ABPs)

Human platelet non-muscle actin APHL99) is randomly labeled on surface lysines with an activated ester of rhodamine;

• Ex: 535 nm, Em: 585 nm – orange/red spectrum
• Labeling stoichiometry: ~0.5 dye per actin monomer.
• Supplied as lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a12% polyacrylamide gel. Rhodamine non-muscle actin was determined to be >99% pure.

The biological activity of rhodamine non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin-down assay. Stringent quality control ensures that >80% of the labeled non-muscle actin can polymerize in this assay.