• In vitro myosin motility assays
• In vitro modeling of the cytoskeleton in the cell cortex
• Study in vivo actin dynamics by labeling of free barbed ends of actin filaments
• Study actin-binding proteins (ABPs)

Rabbit skeletal muscle actin AKL99 is labeled on random surface lysines with an activated ester of rhodamine:

• Ex: 545 nm, Em: 585 nm – orange/red spectrum
• Labeling stoichiometry: ~0.8-1.5 dye per actin monomer.
• Supplied as lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a12% polyacrylamide gel. Purity was determined to be >99% pure

The biological activity of rhodamine actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin-down assay. Stringent quality control ensures that ≥90% of the labeled actin can polymerize.