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Smooth muscle actin has been purified from chicken gizzard—the isotype composition of chicken gizzard smooth muscle actin isapproximately 80% γ-smooth-muscle and 20% β-cytoplasmic-actin.
Chicken gizzard smooth muscle actin has an approximate molecular weight of 43 kDa and is provided as a white lyophilized powder.
Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a12% polyacrylamide gel. Purity was determined to be >99% pure
The biological activity of non-muscle actin is assessed by its ability to efficiently polymerize into filaments in vitro, which can be separated from unpolymerized components using a spin-down assay. Rigorous quality control ensures that >85% of the non-muscle actin polymerizes under these conditions.
Cat. #AS99
