• Detect endogenous phosphorylation in your protein of interest (POI)
• Discover novel regulatory mechanisms for your POI
• Confirm phosphorylation data generated through transfected POI
• Confirm phosphorylation data generated through proteomic approaches
• Discover novel biomarkers

Kit contents (10 - 30 assays)

• BlastR® lysis buffer
• BlastR® dilution buffer
• BlastR-2® wash buffer
• Tyrosine phosphatase inhibitor
• Protease inhibitor cocktail
• Phosphotyrosine affinity beads
• Control beads
• Anti-phosphotyrosine-HRP antibody
• Precision Red™ advanced protein assay reagent
• Bead elution buffer
• DMSO
• Chemiluminescent detection reagents
• Spin column collection tubes
• BlastR® filters

Equipment & materials required

• Cell line or tissue
• Cell scrapers
• Liquid nitrogen for snap freezing lysates
• SDS-PAGE & Western blot equipment
• Primary antibody to POI
• Secondary antibody

The Signal-Seeker® phosphotyrosine detection kit uses affinity beads to enrich phosphotyrosine-modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard Western blot procedures, and the modified protein of interest is detected by the end-user using their primary antibody.

Key characteristics

• Sensitivity: Demonstrated to detect endogenous phosphotyrosine modification of multiple target POI
• Input requirements: 200 µg – 1 mg of lysate
• Timeframe: Approximately 10 hours, typically performed over two days