Tubulin glycerol buffer: cushion buffer

• Supplement for the general tubulin buffer to enhance tubulin polymerization to microtubules
• Compatible with in vitro studies of tubulin/microtubule-associated proteins
• Compatible with tubulin proteins from a wide range of species
• Can be used undiluted as a tubulin cushion buffer, which is used in some assays to separate polymer from unpolymerized tubulin

Supplied as a liquid. Composition: 1x working solution: 80 mM PIPES pH 7.0, 2 mM magnesium chloride, 0.5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), 60% glycerol.

Storage and handling

• BST05 is stable when stored at 4°C
• Supplementation with 1mM guanosine triphosphate (GTP) BST06 is required to create an optimal tubulin buffer. As GTP is labile and will hydrolyze, GTP should be added freshly before use. The GTP-supplemented buffer should not be stored.

All reagents used in this buffer are ≥ American Chemical Society (ACS) grade and ≥99% pure

The biological activity of the BST05 buffer is confirmed by its ability to support in vitro tubulin polymerization.