• Study in vitro regulation of G12V-K-Ras4B cell signaling mechanisms

K-Ras is a small GTP-binding protein that acts as a molecular switch, regulating cell growth, differentiation, and survival through key signaling pathways. Mutations in K-Ras, including the G12V constitutively active mutant, are common in various cancers, leading to uncontrolled cell proliferation and tumor development.

Constitutively active human G12V-K-Ras4B protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: mwt 23-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of G12V-K-Ras4B can be determined by its ability to mimic GTPγS-loaded KRas4B in a Ras activation assay.