• Study in vitro regulation of R-Ras cell signaling mechanisms
• Serves as a substrate for R-Ras GEF assays and drug screens

Although structurally similar to classical Ras proteins, R Ras engages distinct downstream pathways—such as integrin activation and focal adhesion dynamics—rather than primarily triggering MAP kinase pathways, and its activity is context-dependent across cell types

Wild-type human R-Ras protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: mwt 23-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of R-Ras is determined from the ability of the RasGRF1 exchange domain to catalyze the exchange of GDP for GTP on R-Ras.