• Study in vitro regulation of G13D-K-Ras4B cell signaling mechanisms

K-Ras is a small GTP-binding protein that acts as a molecular switch, regulating cell growth, differentiation, and survival through key signaling pathways. Mutations in K-Ras, including the G12V constitutively active mutant, are common in various cancers, leading to uncontrolled cell proliferation and tumor development.

The K-Ras4B mutant K-Ras4B G13D is commonly found in colorectal cancers and is a constitutively active form of K-Ras.

Human K-Ras4B G13D protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: mwt 23-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris, pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of G13D K-Ras4B is determined from the ability of the SOS1 exchange domain (SOS1-ExD) to catalyze the exchange of GDP for GTP onto mutant K-Ras4B.