• Identification/characterization of proteins or small molecules that affect dynein/microtubule interactions
• In vitro analysis of dynein functions

Cytoplasmic dynein is purified from porcine brain by the procedure of B.M. Paschal et al.

The preparation contains;

• Dynein heavy chain (520 kDa)
• Intermediate chain (54, 59, and 74 kDa)
• Light chains (c.20 kDa).

CS-DN01 is supplied as a lyophilized powder

Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. CS-DN01 is ≥80% pure

The biological activity of cytoplasmic dynein is assessed by the ability of microtubules to increase dynein ATPase activity by 5-8 fold.