Activation Assay FAQs
Question 1: Which is the best Small G-protein activation assay for my tissue?
Question 2: Are there any citations for the G-LISA series?
Question 1: Which is the best Small G-protein activation assay for my tissue?
Answer 1: Cytoskeleton, Inc. offers a large number of G-LISA and traditional pull-down activation assays to study the biology and biochemistry of small G-proteins. Our G-LISA activation assays provide an improved method of measuring the activity of small G-proteins utilizing a simple and quick protocol in 96-well format to provide extremely accurate results. To complement our G-LISA line of activation assays, we also offer the most efficiently designed and complete traditional pull-down activation assays available.
The choice of assays for measuring small G-protein activation levels is based on three factors:
For an interactive guide click on one of the hyperlinks.
Our G-LISA activation assays utilize a 96 well format with 12 x 8 well strips that provide the flexibility to run 2 to 96 wells (each well is a condition/treatment) at one time. This flexibility is especially important if any concentration, dose or time course analyses will be performed. Pull-downs are limited to the number of wells in each gel (usually 10-15) that will be run. The G-LISA assays are also more accurate and quantitative than pull-downs with greater sensitivity while using less material per well. The isotype specificity depends on the antibodies being used to capture and visualize the small G-proteins. We have designed both types of activation assays to specifically target RhoA and Rac1. Each of these assays can be easily modified to study RhoB, RhoC, Rac2 or Rac3. For a quick “look and see” experiment with a few treatment conditions (one drug concentration and time point), pull-downs are convenient.
Question 2: Are there any citations for the G-LISA series?
Answer 2: Yes, the G-LISA activation assays are well-cited in the scientific literature. Please see the “citations” tab above for links to some current citations for our RhoA, Rac and Cdc42 G-LISA activation assays.
