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Far-red Actin and Tubulin live cell imaging reagents
The new far-red SiR700 live-cell imaging probes developed by Spirochrome are cell-permeable compounds which stain microtubules (SiR700-Tubulin), F-actin (SiR700-Actin) and chromosomal DNA (SiR700-DNA) in living cells. These stains can be used for dual imaging in conjunction with Spirochrome's existing imaging probes. Spirochrome's live cell imaging probes were originally introduced in a landmark papers published in Nature Methods and Nature recently featured on the cover of the Journal of Biological Chemistry.
- New Wavelengths for Dual Imaging
- Cell Permeable
- Very Low Background
- Far-Red Fluorophore
Spirochrome 700 Series Products
Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.
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Spirochrome technology is based on the proprietary fluorophore silicon rhodamine (SiR). SiR is a bright and photostable rhodamine-like dye. Its key features are its cell permeability, fluorogenic character and compatibility with super-resolution microscopy The fluorescence excitation and emission of SiR are in the far-red, reducing phototoxicity in live-cell imaging experiments. SiR is compatible with most microscopes as it can be used with standard Cy5 settings. The combination of all these properties set SiR-based probes apart from other fluorescent probes. Read more in this Nature Chemistry paper about the properties of SiR.
Fluorogenic probes for live-cell imaging of the cytoskeleton
SiR-actin and SiR-tubulin were recently introduced in a landmark paper published in Nature Methods. The probes combine minimal cytotoxicity with excellent brightness for fluorescence imaging of actin and tubulin. Combined with super-resolution microscopy, SiR-actin and SiR-tubulin permit live-cell imaging of the cytoskeleton with unprecedented resolution.
SiR-actin and SiR-tubulin can be used without transfection and without washing steps. An experiment that highlights these features is the use of SiR-actin to stain the actin fibers of erythrocytes by simply adding SiR-actin to whole blood. Furthermore, their far-red excitation and emission spectra make them compatible with genetically encoded reporters.
"Fluorogenic probes for multi-color imaging in living cells." Lukinavicius G. et al. 2016. J. Am. Chem. Soc. Doi: 10.1021/jacs.6b04782.
“Fluorogenic probes for live-cell imaging of the cytoskeleton”; G. Lukinavičius, L.Reymond, E. D’Este, A. Masharina, F. Göttfert, H. Ta, A. Güther, M. Fournier, S. Rizzo, H. Waldmann, C. Blaukopf, C. Sommer, D. W. Gerlich, H.-D. Arndt, S. W. Hell & K. Johnsson; Nature Methods 11, 731–733, 2014.
“STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons”; E. D’Este, D. Kamin, F. Göttfert, A. El-Hady, S. W. Hell; Cell Reports , Volume 10 , Issue 8 , 1246 – 1251, 2015.
“A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; G. Lukinavičius, K. Umezawa, N. Olivier, A. Honigmann, G. Yang, T. Plass, V. Mueller, L. Reymond, I. R. Corrêa Jr, Z. Luo, C. Schultz, E. A. Lemke, P. Heppenstall, C. Eggeling, S. Manley & K. Johnsson; Nature Chemistry 5, 132–139, 2013.
“Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane”; D. S. Gokhin, R. B. Nowak, J. A. Khoory, A. de la Piedra, I. C. Ghiran and V. M. Fowler; Mol. Biol. Cell; February 25, 2015.
“A cleavable cytolysin-neuropeptide Y bioconjugate enables specific drug delivery and demonstrates intracellular mode of action”; V. M. Ahrens, K. B. Kostelnik, R. Rennert, D. Böhme, S. Kalkhof, D. Kosel, L. Weber, M. von Bergen and A. G. Beck-Sickinger; J. Control. Release; 209:170-178, 2015.
“Red Si–rhodamine drug conjugates enable imaging in GFP cells”; E. Kim, K. S. Yang, R. J. Giedt and R. Weissleder; Chem. Commun., 50, 4504-4507, 2014.
“A marginal band of microtubules transports and organizes mitochondria in retinal bipolar synaptic terminals”; M. Graffe, D. Zenisek, and J. Taraska; J. Gen Physiol. Vol. 146 No.1: 109-117, 2015.
“A Bright Dye for Live-Cell STED Microscopy”; S. Pitsch, I. Köster.
- New BlastR Filtration System
- New Signal-Seeker™ Kits
- New Live Cell Lysosome/Endosome Dyes by Spirochrome AG.
- New Far-red Live Cell Imaging Reagents
- New Antibodies & Reagents
- New Equipment
- Tubulin & FtsZ Products
- Actin Products
- Activation Assays
- Bulk Orders
- Custom Services
- ECM Proteins
- GOBlot - The First Affordable Western Blot Processor
- Kits & Assays
- Live Cell Imaging Reagents
- Motor Proteins
- Protein Assays
- Protein Tools
- Other Proteins
- Signal Seeker Kits
- Small G-proteins
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