• Study mechanisms of Rho family GAP regulation
• Positive control for characterization of Rho GAPs

p50RhoGAP is a regulatory protein that accelerates the GTP hydrolysis of Rho family GTPases, effectively turning off their signaling activity to maintain cytoskeletal balance. It plays a key role in controlling cell shape, motility, and adhesion by modulating actin filament organization.

Wild-type human p50RhoGAP protein is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag for purification
• Molecular weight: approximate mwt 78 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 2 mM Tris, pH 7.5, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥70% pure.

The biological activity of GAP01 is evaluated by the ability of the protein to catalyze GTP hydrolysis by RhoA and Rac1 proteins. Under the experimental conditions used (see datasheet), RhoA GTPase activity is enhanced ≥20 fold in the presence of RhoGAP.