• Study mechanisms of Rho family regulation
• Positive control for characterization of Rho GAPs

Rho GDP dissociation inhibitor (RhoGDI) is a regulatory protein that controls the cycling of Rho family GTPases between active and inactive states, maintaining them in the cytosol in their GDP-bound form. By modulating Rho GTPase signaling, RhoGDI plays a key role in cytoskeletal organization, cell motility, and intracellular trafficking.

The human Rho guanine nucleotide dissociation inhibitor protein (RhoGDI) is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag for purification
• Molecular weight: approximate mwt 57 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 2 mM Tris, pH 7.5, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.