Profilin 1 protein: His tagged: human
Product Uses Include
- Actin binding studies
- Actin polymerization studies
Profilin protein has been purified from human platelets. The protein is supplied as a lyophilized powder. When resuspended in dH2O, the profilin will be in the following buffer: 100 mM Tris pH 7.4, 100 mM NaCl, 0.2 mM ATP, 5% sucrose and 1% dextran. The lyophilized protein is stable at 4°C desiccated (<10% humidity) for at least 2 years.
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Purity is >95% (see figure 1).
|Figure 1. Profilin purity determination. A 5.0 µg sample of PR01 (profilin molecular weight approx. 15 kDa) was run on an SDS-PAGE gel and stained with coomassie blue. Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat.# ADV02).||
Profilin is tested for biological activity by a binding assay with actin. Profilin is bound to poly-L-proline sepharose beads and incubated with G-actin. The profilin beads bind >90% of the available actin by this procedure.
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Moseley, J. B., Sagot, I., Manning, A. L., Xu, Y., Eck, M. J., Pellman, D. and Goode, B. L. (2004). A conserved mechanism for Bni1- and mDia1-induced actin assembly and dual regulation of Bni1 by Bud6 and profilin. Mol. Biol. Cell 15, 896-907.
Vignjevic, D., Yarar, D., Welch, M. D., Peloquin, J., Svitkina, T. and Borisy, G. G. (2003). Formation of filopodia-like bundles in vitro from a dendritic network. J. Cell Biol. 160, 951-962.
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