Cytoskeleton provides a range of antibodies raised to the Rho family of small G-proteins. They have exceptional isoform specificity and have been quality checked by Western blot, immuno-fluorescence in situ, ELISA and immuno-precipitation in the QC Max protocol. For more information on each antibody please click on the pdf icon below to see the datasheet.
In addition, Cytoskeleton has developed a unique line of fluorescent phalloidins with improved brightness and stability compared to other conjugates such as Alexa fluor and Cy dyes, for more information see the Acti-staininformation page.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at firstname.lastname@example.org.
Cytoskeleton's antibodies products have been cited hundreds of times over the past 18 years. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.
Anti-Cdc42: mouse monoclonal (Cat. # ACD03)
Giordano Pula and Alastair W. Poole. Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin a2b1. Platelets, 2008, Vol. 19, No. 3, Pages 199-210 , DOI: 10.1080/09537100701777303
Akio Shimizu and Akiko Mammoto et al. ABL2/ARG Tyrosine Kinase Mediates SEMA3F-induced RhoA Inactivation and Cytoskeleton Collapse in Human Glioma Cells. Journal of Biological Chemistry, 283, 27230-27238. DOI: 10.1074/jbc.M804520200
Anti-RhoA: mouse monoclonal (Cat. # ARH03)
Khan et al. (2011). Geranylgeranyltransferase type I (GGTase-I) deficiency hyperactivates macrophages and induces erosive arthritis in mice. J Clin Invest doi:10.1172/JCI43758.
Question 1: How do you measure the isoform specificity of your antibodies?
Answer 1: Isoform specificity of our small G-protein antibodies is measured through a rigorous western blot screening process using purified small G-proteins from the entire Rho family to insure that each antibody is specific for its intended protein target. The RhoA specific antibody (Cat. # ARH03) has been tested against not only RhoB and RhoC, but also Ras-H, Rac1 and Cdc42 proteins. ARH03 will not cross react with ten-fold excess of RhoB, RhoC, Ras-H, Rac1 or Cdc42 proteins. Most commercially available Rac1 antibodies also recognize Rac2, Rac3 and/or Cdc42 proteins. Our Rac1 specific antibody (Cat. # ARC03) has been tested against Rho, Rac2, Rac3 and Cdc42 proteins and only detects Rac1 protein. Similarly, the Cdc42 specific antibody (Cat. # ACD03) has been tested against Rac and Rho proteins. ACD03 will not cross react with a ten-fold excess of RhoA, RhoB, RhoC, Rac1, Rac2 or Rac3 proteins. For ACD03, non-specific bands have been observed at higher molecular weight in about 50% of cell lines tested.
Question 2: What antibody affinity is good for western blots and ELISAs?
Answer 2: Optimal antibody affinities differ based on the experimental format being utilized. For western blotting, an antibody dilution range of 1:100 to 1:10,000 from a 1 mg/ml stock is typical. Another way to express these values is a Molarity concentration along a log scale. For western blotting, the useful antibody affinity (Kd) is 10-4 to 10-12 M. For ELISAs, the useful Kd range is 10-8 to 10-12 M.