ATPase & GTPase Assays

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com.

Many publications cite the use of Cytoskeleton's kits in the Materials and Methods section of papers. Usually the citation is associated with a particular result in the form of a graph or image that helps the you, the authors, present your findings. This indicates the utility of the Kits to produce publication quality data in a short timeframe thus helping improve the productivity of your efforts. Example citations for phosphate assay kits are shown below.  More citations are available on individual product pages.



Question 1:  My GTPase has a low GTPase activity.  Can I still use it in an assay?

Answer 1: Yes, Cytoskeleton offers the EasyRad Phosphate Assay Biochem Kit (Cat. # BK055) that is specifically designed for low activity GTPases.  The kit is a simple two step assay based on the separation of ^{32 or 33}Phosphate from ^{32 or 33}Gamma-Phosphate-Nucleoside Triphosphate (NTP).  This is the highest sensitivity phosphate assay available, allowing K_cat measurements as low as 0.00001 (NTPs hydrolysed per sec per molecule). 

Question 2:  What is the smallest amount of test protein that I can use in the kinetic ATPase assay (Cat.# BK051)?

Answer 2: We recommend preparing the test protein at a concentration of 1 mg/ml if purity is >90%.  If the protein is at lower purity, we recommend increasing the total protein concentration to compensate for the lower purity. The protein must be in a phosphate-free buffer such as Tris-HCl (PBS is not suitable).  We strongly recommend performing a protein titration. A good titration range is between 0.1 μg – 20 μg per assay.

For more information, click on the Documents tab above and see the datasheets, or contact Technical Support at tservice@cytoskeleton.com.