Proteins

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com.

Cytoskeleton's motor protein products have been cited hundreds of times over the past 18 years. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.



Question 1:  What is a good control compound for inhibiting kinesin motor proteins?

Answer 1:  Unfortunately, only a limited number of kinesin inhibitors exist.  A general ATP-site inhibitor can be a non-hydrolyzable ATP analog.  These give excellent inhibition as presented in Funk et al., 2004 (Development of high-throughput screens for discovery of kinesin adenosine triphosphatase modulators. Anal Biochem. 329, 68-76).  Another well-utilized inhibitor is monastrol which inhibits Eg5.  Monastrol is a reversible, cell-permeable, non-tubulin interacting inhibitor of the mitotic kinesin Eg5 motor protein.  In some situations the antibodies to the motor proteins can be used to inhibit motor functions.  This would need to be determined empirically.

Question 2:  Do you sell any full length kinesins or ones that have microtubule motility?

 Answer 2: Unfortunately, we do not sell any full length kinesins.  However, we sell many purified kinesin motor domain proteins, including Cat. # CP01(CENP-E), CR01 (Chromokinesin), EG01(Eg5), BM01 (Eg5 homolog BimC from A. nidulans), EG02 (Eg5 homolog BimC from A. fumigatus), KC01 (KIFC3), KF01 (KIF3C), KR01 (kinesin heavy chain), MK01 (MCAK) and MP01 (MKLP).  For microtubule motility studies, we recommend kinesin heavy chain motor domain protein (Cat. # KR01).  We offer this motor protein, rhodamine-labeled tubulin, unlabeled tubulin, antifade solution, ATP (Cat. # BSA04-001), microtubule cushion buffer, general tubulin buffer (Cat. # BST01-001), blocking solution, chamber wash buffer, kinesin buffer, taxol (Cat. # TXD01), DMSO, perfusion chamber filter paper and acid-washed perfusion chambers (Cat. # BSM05-04) in a convenient kit format (Cat. # BK027).   The kinesin heavy chain motor (Cat. # KR01) can serve as a positive control in this kit when studying an uncharacterized motor protein or as a substrate to examine how a drug/compound affects kinesin/microtubule motility.

For more information, click on the Documents tab above to see the datasheet, or contact Technical Support at tservice@cytoskeleton.com.