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Arf1 and Arf6 Activation Assays and
GTPase Affinity Beads

Cytoskeleton’s Arf1 and Arf6 activation assays are in two formats: G-LISAs and pull-downs. G-LISAs use a 96 well plate coated with an effector protein that selectively binds active Arf1 or Arf6, followed by quantitation with ELISA techniques. Typical Arf6 G-LISA data are shown (right). Arf pull-downs utilize the Arf1 and Arf6 protein binding domain (PBD) of the effector protein GGA3 (Golgi-localized γ-ear containing, Arf-binding protein 3), which specifically binds the GTP-bound form of Arf1 and Arf6. The GGA3-PBD is covalently conjugated to colored sepharose beads, enabling the “pull-down”of active Arf1-GTP and Arf6-GTP for quantification by Western blotting.
Right: Arf6 activation measured by G-LISA. Lysates for Arf6 G-LISA were prepared from MDCK cells that were either attached to tissue culture plates (activated) or kept in suspension for 2 hours (control). 12.5, 6.25, and 3.1 ugs of cell lysates were subjected to the G-LISA assay (Cat. # BK133). Absorbance was read at 490 nm. Data are background subtracted.
New Products
- Arf1 G-LISA Activation Assay Biochem Kit (Cat. # BK132)
- Arf1 Activation Assay Biochem Kit (Pull-down format) (Cat. # BK032-S)
- Arf6 G-LISA Activation Assay Biochem Kit (Cat. # BK133)
- Arf6 Activation Assay Biochem Kit (Pull-down format) (Cat. # BK033-S)
- GGA3-PBD Beads (Cat. # GGA05)
For more information about the Arf small G-protein click here.
Select from the categories below:
In response to customers' requests, Cytoskeleton Inc. has reciently developed the products shown below:
New Products
- Arf1 G-LISA Activation Assay Biochem Kit (Cat. # BK132)
- Arf1 Activation Assay Biochem Kit (Pull-down format) (Cat. # BK032-S)
- Arf6 G-LISA Activation Assay Biochem Kit (Cat. # BK133)
- Arf6 Activation Assay Biochem Kit (Pull-down format) (Cat. # BK033-S)
- GGA3-PBD Beads (Cat. # GGA05)
Cytoskeleton, Inc. was formed in 1993. Since the start we have continuously been expanding our line of products. Because our scientists have many years of experience working in their respective areas of expertise, we are able to provide products of exceptionally high quality. Since the start in 1993 we have gained a reputation for producing quality products at a reasonable price. Indeed, our products have been used in many hundreds of scientific publications. See individual product pages for example citations.
If there is a particular product that you would like to see commercially available please do not hesitate to contact us. We are here to help.
Cytoskeleton's products have been cited hundreds of times over the past 18 years. As these products are new, scientists have not yet had the time to publish with these materials.
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