Fibrillarin nuclear marker has been used for many years to identify the nucleoleus in fixed cells. Cytoskeleton provides the monoclonal antibody originally develop By Dr. Eng Tan's lab.
Reimer, G., K. M. Pollard, C. A. Penning, R. L. Ochs, M. A. Lischwe, H. Busch, and E. M. Tan. 1987. Monoclonal autoantibody from a (New Zealand black x New Zealand white) F1 mouse and some human scleroderma sera target and Mr 34,000 nucleolar protein of the U3 RNP particle. Arthritis Rheum. 30: 793-800.
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Cytoskeleton's products have been cited hundreds of times over the past 18 years. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.
Anti-Fibrillarin (nuclear marker, Cat. # AFB01)
Arabi, A., Wu, S., Ridderstrale, K., Bierhoff, H., Shiue, C., Fatyol, K., Fahlen, S., Hydbring, P., Soderberg, O., Grummt, I. et al. (2005). c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription. Nat. Cell Biol. 7, 303-310.
Orihara-Ono, M., Suzuki, E., Saito, M., Yoda, Y., Aigaki, T. and Hama, C. (2005). The slender lobes gene, identified by retarded mushroom body development, is required for proper nucleolar organization in Drosophila. Dev. Biol. 281, 121-133.
Arabi, A., Rustum, C., Hallberg, E. and Wright, A. P. (2003). Accumulation of c-Myc and proteasomes at the nucleoli of cells containing elevated c-Myc protein levels. J. Cell Sci. 116, 1707-1717.
Gonda, K., Fowler, J., Katoku-Kikyo, N., Haroldson, J., Wudel, J. and Kikyo, N. (2003). Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b. Nat. Cell Biol. 5, 205-210.
Polak, P. E., Simone, F., Kaberlein, J. J., Luo, R. T. and Thirman, M. J. (2003). ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia. Mol. Biol. Cell 14, 1517-1528.
Rubbi, C. P. and Milner, J. (2003). Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses. EMBO J. 22, 6068-6077.
Sugimoto, M., Kuo, M. L., Roussel, M. F. and Sherr, C. J. (2003). Nucleolar Arf tumor suppressor inhibits ribosomal RNA processing. Mol. Cell 11, 415-424.
Caballero, O. L., Resto, V., Patturajan, M., Meerzaman, D., Guo, M. Z., Engles, J., Yochem, R., Ratovitski, E., Sidransky, D. and Jen, J. (2002). Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Oncogene 21, 3003-3010.
Question 1: What is the nuclear structure that is recognized by the anti-fibrillarin antibody?
Answer 1: Cytoskeleton's anti-fibrillarin antibody (Cat. # AFB01) labels fibers that occur around the nucleoleus which is an internal structure of a nucleus.