Cat. # PR01

Product Uses Include

• Actin binding studies
• Actin polymerization studies
  
  

Material
Profilin protein has been purified from human platelets. The protein is supplied as a lyophilized powder. When resuspended in dH2O, the profilin will be in the following buffer: 100 mM Tris pH 7.4, 100 mM NaCl, 0.2 mM ATP, 5% sucrose and 1% dextran. The lyophilized protein is stable at 4°C desiccated (<10% humidity) for at least 2 years.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Purity is >95% (see figure 1).

Figure 1. Profilin purity determination. A 5.0 µg sample of PR01 (profilin molecular weight approx. 15 kDa) was run on an SDS-PAGE gel and stained with coomassie blue. Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat.# ADV02).

Biological activity
Profilin is tested for biological activity by a binding assay with actin. Profilin is bound to poly-L-proline sepharose beads and incubated with G-actin. The profilin beads bind >90% of the available actin by this procedure.