Cat. # AS99

Product Uses Include

• Identification and characterization of smooth muscle actin binding proteins
• In vitro smooth muscle actin polymerization studies
  
  

Material:
Smooth muscle actin has been purified from chicken gizzard.  The isotype composition of chicken gizzard smooth muscle actin is approximately 80% γ smooth muscle and 20% β cytoplasmic actin (1).  Chicken gizzard smooth muscle actin has an approximate molecular weight of 43 kDa and is provided as a lyophilized white powder.

Purity:
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel.  Smooth muscle actin is >99% pure (see Figure 1).

Figure 1. Smooth Muscle Actin Protein Purity Determination.  A 100 µg sample of chicken gizzard smooth muscle actin (molecular weight approx.  43 kDa) was separated by electrophoresis in a 12% SDS-PAGE system, and stained with Coomassie Blue.  Protein quantitation was determined with the Precision Red Protein Assay Reagent (Cat. # ADV02).

Biological Activity:
The biological activity of smooth muscle actin is determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay.  Stringent quality control ensures that >85% of the smooth muscle actin polymerizes in this assay.

References

1. North, A. J. et al. 1994. J. Cell Sci. 107, 445-455