Cat. # ACD03

Introduction

Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.

Product Uses Include

This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts.

The following protocols have been tested with this antibody: 

*     Western blot analysis:  recommended 

*     Immunoprecipitation:  not recommended  

*     Immunocytochemistry:  recommended with pre-test on western blot

Material

Figure 1 - Western analysis of small G-proteins and cell extracts.

Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane.  ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.

Table 1 - Reactivity of other commercially available Cdc42 antibodies

NT = Not tested

Examples of publications where this product was cited:

Giordano Pula and Alastair W. Poole. Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin a2b1.

Platelets, 2008, Vol. 19, No. 3, Pages 199-210 , DOI: 10.1080/09537100701777303

Akio Shimizu and Akiko Mammoto et al. ABL2/ARG Tyrosine Kinase Mediates SEMA3F-induced RhoA Inactivation and Cytoskeleton Collapse in Human Glioma Cells.

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