Cat. # ARH03

Introduction

RhoA is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. RhoA is the predominant Rho isoform that is expressed in a large number of different cell types.

Product Uses Include

This antibody is recommended for detection of RhoA in human, mouse, rat or other extracts.

The following protocols have been tested with this antibody: 

*     Western blot analysis:  recommended 

*     Immunoprecipitation:  not recommended  

*     Immunocytochemistry:  recommended with pre-test on western blot

Material

Figure 1 - Western analysis of small G-protein sub-families.

Figure 1: Western blot analysis of purified small G-proteins using ARH03 as the primary antibody.. Recombinant small G-proteins and platelet extract were separated by SDS-PAGE and transferred to PVDF membrane.  Anti-RhoA antibody was diluted to 250 ng/ml (1:1000) in PBST and the signal developed with a sensitive chemiluminescence reagent. Lane 1; 25 ng RhoA-6xHIS, Lane 2; 250 ng RhoB-6xHIS, Lane 3; 250 ng RhoC-6xHIS, Lane 4; 250 ng Rac1-6xHIS, Lane 5; 250 ng Cdc42-6xHIS, Lane 6; 40 µg human platelet extract. Note: RhoA-6xHIS runs at 28kDal whereas the native RhoA in platelet extract runs at 23kDal.

Figure 2 - Western analysis of cell extracts from different species

Figure 2: Western blot analysis of cell extracts using ARH03 as the primary antibody.  Chemiluminescence detection of  RhoA in cell extracts (50 µg each) of rat NRK cells (lane 1), human HeLa cells (lane 2), bovine brain extract (lane 3), and human platelet cell extract (lane 4). The RhoA band is located at approx. 22kDal.  The blot was probed with a 250 ng/ml (1:1000) dilution of anti-RhoA antibody.  Molecular weight markers are from Invitrogen.

Table 1 - Reactivity of other commercially available RhoA antibodies

NT = Not tested

Examples of publications where this product was cited:
This product was introduced December 2007, as yet there are no citations for this product.