Biochem™ kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays in cytoskeletal research. Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers. Cytoskeleton Inc provides Biochem kits for actin, tubulin, intermediate filament, G-protein, signal transduction and molecular motor research.
If you are new to the field of cytoskeletal research, the Biochem™ kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If either of these points are overlooked, the results could lead to inappropriate experimental interpretation. Biochem™ kits are designed to overcome this bottleneck in research.
If you wanted to know whether a specific protein or compound interacts with actin filaments and/or monomer, you could order one of several Biochem kits specializing in actin research:
1) Actin binding protein assay Biochem™ kit (cat # BK001): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin filaments. It should be noted that in some cases actin binding proteins have a low affinity for actin filaments. Therefore, it is wise to couple this assay with the Actin polymerization assay Biochem™ kit in order to obtain a more complete profile of your potential actin binding protein.
2) Actin filament assay Biochem™ kit (cat. # BK002): This is a simple kit that allows researchers to reproducibly obtain actin filaments and/or actin monomer to be used as substrates for actin research.
3) Actin polymerization assay Biochem™ kit (cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on actin polymerization. Cytoskeleton has adapted this powerful assay into a user friendly Biochem™ kit format.
Biochem™ kits are also a great way to optimize your research time and money. When you buy a Biochem™ kit, you obtain all the necessary proteins, buffers, controls and instructions that you require to perform your research with the highest degree of confidence. Furthermore, Cytoskeleton Inc guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.
