Cat. # BK007R

Product Uses Include

• Create fluorescent microtubules for in vitro motility assays.
• Use separate components for separate experiments (e.g. microinjection and biochemical assays).
• Teaching aid for undergraduate or graduate classes.
  
  

Introduction
The contents of this kit will allow you to reproducibly prepare fluorescent microtubules of a predetermined mean length and of a predetermined fluorescent intensity. After polymerization the microtubules can be used directly or stabilized with taxol before use, depending upon the needs of your experimental system. This kit is ideal for the preparation of taxol stabilized fluorescent microtubules for use in an in vitro motility assay.

Figure 1. Rhodamine labeled microtubules created with kit BK007R

Kit contents
The kit contains enough materials for 50-200 assays depending on experimental setup. The following components are included:

1. 5 x 20 µg Rhodamine Labeled Tubulin (Cat. # TL331M).
2. 2 x 250 µg Tubulin Plus Glycerol (Cat. # TL238).
3. General Tubulin Buffer (Cat. # BST01).
4. GTP in PEM Buffer (Cat. # BST06).
5. Tubulin Glycerol Buffer (Cat. # BST05).
6. Antifade
7. Manual with detailed protocols and extensive troubleshooting guide
   
   

1. Fluorescence microscope equipped with filters for rhodamine detection