Biochem Kits™
PhosFree™ phosphate assay

Cat. # BK050

Product Uses Include

  • To measure phosphate in a biological solution or buffer
  • To measure ATP or GTP hydrolysis from an enzyme reaction
  • To measure phosphate release from phosphatase catalyzed reactions

Introduction
The PhosFree™ phosphate assay Biochem Kit™ is an extremely quick and economical way to measure phosphate in solution. There is minimal interference from other common ions and reagents used in molecular biology and biochemistry. The manual designed to guide you through the process of measuring end-point and kinetic assays. There is special consideration for ATPase and GTPase assays, which can be performed easily with this kit.

The PhosFree™ phosphate assay is specifically developed for measuring phosphate in high protein concentration solutions (>0.5 mg/ml). For measuring phosphate in lower protein concentration solutions, Cytoskeleton, Inc. recommends using the CytoPhos™ phosphate assay (Cat. # BK054).

Kit contents
The kit contains sufficient materials for 1000 assays. The following components are included:

  1. Activator.
  2. Quencher
  3. 1 mM Phosphate Standard in dH2O
  4. PhosFree™ Phosphate Assay Manual

Equipment needed

  1. Spectrophotometer capable of measuring absorbance at 650 nm wavelength.
  2. Small capacity (100-1000 µl) cuvettes or 96-well microtiter plates.

Examples of publications where this product was used
Lee, B. I., Kim, K. H., Park, S. J., Eom, S. H., Song, H. K. and Suh, S. W. (2004). Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair. EMBO J 23, 2029-2038.
Product description Cat. # Amount Price & Order
PhosFree™ phosphate assay BK050 1000 assays