Cat. # TXD01
Product Uses Include
- Promote tubulin polymerization in vitro
- Promote microtubule stability in vitro
- Anti-mitotic drug in cell cultures
Material
Paclitaxel's (Taxol) usefulness as a laboratory tool is well established and lies in its ability to inhibit microtubule depolymerization. Thus, taxol stabilized microtubules are used as substrates for the identification and characterization of the ever increasing number of microtubule associated proteins.
Paclitaxel is provided as a lyophilized powder. DMSO for resuspension is supplied with the product. When resuspended the paclitaxel is at 2 mM concentration.
Purity
In keeping with our policy of providing only the highest quality products, we are now pleased to inform you that Cytoskeleton is offering paclitaxel (taxol), from the Pacific yew tree, Taxus brevifolia, at a chromatographic purity of >99.5%. This is the purest taxol commercially available for research use.
Biological Activity
We have determined that the microtubule stabilizing property of our product is equal or superior to any other commercially available taxols. Microtubules in 10 µM paclitaxel are stable at room temperature for over one week.
Examples of publications where this product was used:
Benink, H. A., Mandato, C. A. and Bement, W. M. (2000). Analysis of cortical flow models in vivo. Mol. Biol. Cell 11, 2553-2563.
Korinek, W. S., Copeland, M. J., Chaudhuri, A. and Chant, J. (2000). Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Science 287, 2257-2259.
Kosturko, L. D., Maggipinto, M. J., D'Sa, C., Carson, J. H. and Barbarese, E. (2005). The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2. Mol. Biol. Cell 16, 1938-1947.
Ligon, L. A., Shelly, S. S., Tokito, M. and Holzbaur, E. L. (2003). The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Mol. Biol. Cell 14, 1405-1417.
Nair, K. S., Hanson, S. M., Kennedy, M. J., Hurley, J. B., Gurevich, V. V. and Slepak, V. Z. (2004). Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. J. Biol. Chem. 279, 41240-41248.
Teckchandani, A. M., Birukova, A. A., Tar, K., Verin, A. D. and Tsygankov, A. Y. (2005). The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules. Exp. Cell Res. 306, 114-127.
Wagner, O. I., Ascano, J., Tokito, M., Leterrier, J. F., Janmey, P. A. and Holzbaur, E. L. (2004). The interaction of neurofilaments with the microtubule motor cytoplasmic dynein. Mol. Biol. Cell 15, 5092-5100
| Product description | Cat. # | Amount | Price & Order |
| Paclitaxel | TXD01 | 10 x 100 µl (2 mM) |
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