Cytoskeleton, Inc. has the widest line of G-protein related products on the market. Cytoskeleton sells purified proteins, a large number of Biochem Kits™ to assay the biology and biochemistry of small G-proteins as well as antibodies to G-proteins and related proteins. See each individual product page for examples of uses.
G-LISA™ Activation Assays:
Cdc42 G-LISA™ Activation Assay, colorimetric format (Cat.# BK127)
Rac1,2,3 G-LISA™ Activation Assay, colorimetric format (Cat.# BK125)
RhoA G-LISA™ Activation Assay, colorimetric format (Cat.# BK124)
RhoA G-LISA™ Activation Assay, luminescence format (Cat.# BK121)
Rac1 G-LISA™ Activation Assay, luminescence format (Cat.# BK126)
Inhibitor
Rho Inhibitor, cell permeable (Cat. # CT04)
Activators
Rho Activator (Cat. # CN01)
Rac and Cdc42 Activator (Cat. # CN02)
Purified Proteins
GTPases
Effector fragments
GTPase activating protein (GAPs)
Guanine nucleotide dissociation inhibitors (GDIs)
Guanine nucleotide exchange factors (GEFs)
Modifying enzymes
Small G-protein and signal transduction Biochem Kits™
Cdc42 G-LISA activation assay (BK127)
EasyRad phosphate assay (BK055)
RhoA activation assay, G-LISA, absorbance based (BK124)
RhoA activation assay, G-LISA, luminometry based (BK121)
RhoA/B/C activation assay, G-LISA, absorbance based (BK123)
Rac1,2,3 activation assay, G-LISA, absorbance based (BK125)
Rac1 activation assay, G-LISA, luminometry based (BK126)
Rac activation assay, pull down (BK035)
Ras activation assay, pull down (BK008)
RhoA activation assay, pull down (BK036)
RhoGAP assay (BK105)
RhoGEF exchange assay (BK100)
Antibodies to G-proteins and associated proteins
Anti-Cdc42 monoclonal antibody (Cat.# ACD03)
Anti-Rac1 monoclonal antibody (Cat.# ARC03)
Anti-RhoA monoclonal antibody (Cat.# ARH03)
Anti-Cofilin rabbit polyclonal (Cat. # ACFL02)
Anti-Profilin rabbit polyclonal (Cat. # APUF01)
Purified proteins
GTPases
Cdc42 protein, GST-tagged, wild type [GST-Cdc42]
Cdc42 protein, His-tagged, wild type [His-Cdc42]
Cdc42 protein, His-tagged, constitutively active [His-Cdc42(Q61L)]
Cdc42 protein, GST-tagged, dominant negative [GST-Cdc42(T17N)]
H-Ras protein, His-tagged, wild type [His-H-Ras]
H-Ras protein, GST-tagged, constitutively active [GST-H-Ras(G12V)]
Rac1 protein, GST-tagged, wild type [GST-Rac1]
Rac1 protein, His-tagged, wild type [His-Rac1]
Rac1 protein, His-tagged, constitutively active [His-Rac1(Q61L)]
Rac1 protein, GST-tagged, dominant negative [GST-Rac1(T17N)]
Ran protein, His-tagged, wild type [His-Ran]
Ran protein, His-tagged, constitutively active [His-Ran(Q69L)]
Ran protein, His-tagged, dominant negative [His-Ran(T24N)]
Rap1a protein, His-tagged, wild type [His-Rap1a]
Rap1b protein, His-tagged, wild type [His-Rap1b]
RhoA protein, GST-tagged, wild type [GST-RhoA]
RhoA protein, His-tagged, wild type [His-RhoA]
RhoA protein, His-tagged, constitutively active [His-RhoA(Q63L)]
RhoC protein, His-tagged, wild type [His-RhoC]
GTP-gamma-S (non-hydrolyzable analog of GTP, for preparing "active conformation" of G-proteins
Effector fragments
PAK p21-binding domain protein, GST-tagged [GST-PAK-PBD]
PAK p21-binding domain protein on agarose beads, GST-tagged [GST-PAK-PBD beads]
Raf Ras-binding domain protein on agarose beads, GST-tagged [GST-Raf-RBD beads]
RanBP1 protein, His-tagged [His-RanBP1]
Rhotekin Rho-binding domain protein, GST-tagged [GST-Rhotekin-RBD]
Rhotekin Rho-binding domain protein on agarose beads, GST-tagged [GST-Rhotekin-RBD beads]
WASP Cdc42-binding domain protein on agarose beads, GST-tagged [GST-WASP-CBD beads]
GTPase activating protein (GAPs)
p50RhoGAP protein, GST-tagged, full length [GST-p50RhoGAP]
p50RhoGAP protein, GST-tagged, catalytic domain [GST-p50RhoGAP, catalytic]
Guanine nucleotide dissociation inhibitors (GDIs)
Rho guanine nucleotide dissociation inhibitor protein-1, GST-tagged [GST-RhoGDI-1]
Guanine nucleotide exchange factors (GEFs)
Dbs RhoGEF domain protein, His-tagged [His-Dbs-DH/PH]
Modifying enzymes
C3 transferase protein
Cell Permeable Rho Inhibitor
