Cat. # RN03

Product Uses Include

• Ran effector and GAP identification and binding studies
• Activation of Ran effectors in vitro and in vivo
• Stimulation of Ran cellular effects by microinjection
  
  

Material
The constitutively active form of human Ran protein contains a glutamine to leucine substitution at residue 69. The common name for this mutant is Ran(Q69L) or L69Ran. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of Ran, hence the protein is always in the active, GTP-bound, state. The corresponding mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.

The recombinant protein is approximately 31 kDa, consisting of the Ran protein plus a histidine tag in the amino-terminus. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.

For other forms of Ran (wild type, dominant negative) as well as many other purified small G-proteins, see our main small G-protein product page.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-Ran samples are >90% pure.

Figure 1: His-Ran(Q69L) protein purity determination. A 10 µg sample of RN03 was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.