Cat. # V01
Product uses
- Investigating vimentin filament dynamics.
- Identification of vimentin associated proteins.
- Vimentin is an excellent substrate for protein kinases.
Material
Recombinant Syrian hamster vimentin has been expressed in and purified from a bacterial expression system. Vimentin is also available in a Vimentin Biochem Kit™ (BK020).
Purity
Purity is determined by scanning densitometry of the protein on an SDS-PAGE gel. Vimentin is >90% pure.
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Figure 1. Vimentin protein purity determination. A 20 µg sample of V01 was separated by electrophoresis in a 12% SDS-PAGE system, and stained with Coomassie Blue. |
Biological Activity
The protein is supplied in a non-polymerized form. Polymerization to form vimentin intermediate filaments (IFs) can proceed by the addition of NaCl to 150 mM final concentration. V01 has a critical concentration 0.017 mg/ml.
Examples of publications where this product was used:
Perlson, E., Hanz, S., Ben-Yaakov, K., Segal-Ruder, Y., Seger, R. and Fainzilber, M. (2005). Vimentin-dependent spatial translocation of an activated MAP kinase in injured nerve. Neuron 45, 715-726.
Styers, M. L., Salazar, G., Love, R., Peden, A. A., Kowalczyk, A. P. and Faundez, V. (2004). The endo-lysosomal sorting machinery interacts with the intermediate filament cytoskeleton. Mol. Biol. Cell 15, 5369-5382.
| Product description | Cat. # | Amount | Price & Order |
| Vimentin protein | V01-A | 2 x 50 µg |
|
| V01-C | 10 x 50 µg |
|
