Cat. # ML116
Product Uses Include
- Studying the effects of MAPs on microtubule dynamics
- Studying the binding sites between MAPs and tubulin.
- Studying the effects of anti-microtubule drugs on microtubule polymerization.
Material
Tubulin and microtubule associated proteins (MAPs) has been purified from porcine brain by an adaptation of the method of Shelanski et al. (1). Tubulin is supplied as a white lyophilized powder. The protein composition is approximately 70% tubulin (55 kDa heterodimer) and 30% MAPs. The MAPs in this product act to stabilize microtubules and to enhance tubulin polymerization. MAP rich tubulin can polymerize efficiently at 1-2 mg/ml.
MAP-rich tubulin is also available from a bovine source (Cat. # ML113).
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are approximately 70% tubulin and 30% MAPs.
Biological Activity
One unit of MAP-rich tubulin is defined as 5.0 mg of protein (as determined by the Precision Red Protein Assay Reagent, Cat. # ADV02). MAP-rich tubulin will polymerize efficiently at protein concentrations above 1mg/ml and temperatures above 24°C.
References
- Shelanski M. L., et al. (1973). Proc. Natl. Acad. Sci. USA. 70, 765-768
| Product description | Cat. # | Amount | Price & Order |
| Porcine MAP rich tubulin (lyophilized) | ML116-A | 1 x 1 mg |
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| ML116-B | 5 x 1 mg |
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| ML116-C | 20 x 1 mg |
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| ML116-DX | 1 x 10 mg |
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| ML116-XL | Large quantities |
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