The BlastR™ lysis filtration system allows users to capture proteins from all cellular compartments using a denaturing buffer to obtain a more complete protein profile relative to standard non-denaturing buffers. One significant drawback with denaturing buffers is the viscous genomic DNA contamination that can interfere with protein analysis, migration of proteins on an SDS-PAGE gel, and interference with immunoprecipitation assays.
BlastR™ filters remove viscous genomic DNA from denaturing cell lysis buffer extracts while reducing processing time to less than one minute. The proprietary filtration technology is based on a compressible polyurethane filter with optimal pore size for capturing genomic DNA. The compressible nature of the filter allows for maximal recovery of sample which is usually 90% of the original volume.
Two versions are available: 1) filters plus buffers version (Cat. #BLR01, includes; BlastR™ Lysis Buffer), which was developed as an integrated component of the Signal-Seeker™ PTM detection product line. The BlastR™ lysis buffer was optimized as a "universal" immunoprecipitation buffer for low abundance PTM modified proteins. Additionally, it effectively isolates proteins from all cellular compartments making it useful for western blot applications. 2) Filters only (Cat. #BLR02, which allows the operator to use their own denaturing buffer such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity.
Click on the items below for more information, or click on the resources tab for technical tips on the BlastR™ lysis filtration system.
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