Cytoskeleton offers GDP and GTP for small G-protein in vitro research. In addition we supply a line of fluorescent phalloidins under the tradename Acti-stain™. For more detailed information click on the datasheet link below.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at firstname.lastname@example.org.
Cytoskeleton's products have been cited hundreds of times over the past 18 years. A select few are described here, for more citations on individual products please use the "Citations" tab on each individual product page.
Phalloidin (rhodamine): 14uM (Cat. # PHDR1)
Ezratty, E. J., Partridge, M. A. and Gundersen, G. G. (2005). Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase. Nat. Cell Biol. 7, 581-590.
Gomes, E. R., Jani, S. and Gundersen, G. G. (2005). Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell 121, 451-463.
Chandhoke, S. K., Williams, M., Schaefer, E., Zorn, L. and Blystone, S. D. (2004). β3 integrin phosphorylation is essential for Arp3 organization into leukocyte αVβ3-vitronectin adhesion contacts. J. Cell Sci. 117, 1431-1441.
Hsieh-Wilson, L. C., Benfenati, F., Snyder, G. L., Allen, P. B., Nairn, A. C. and Greengard, P. (2003). Phosphorylation of spinophilin modulates its interaction with actin filaments. J. Biol. Chem. 278, 1186-1194.
Qian, Y., Baisden, J. M., Cherezova, L., Summy, J. M., Guappone-Koay, A., Shi, X., Mast, T., Pustula, J., Zot, H. G., Mazloum, N. et al. (2002). PKC phosphorylation increases the ability of AFAP-110 to cross-link actin filaments. Mol. Biol. Cell 13, 2311-2322.
Chen, J., Fabry, B., Schiffrin, E. L. and Wang, N. (2001). Twisting integrin receptors increases endothelin-1 gene expression in endothelial cells. Am J Physiol Cell Physiol 280, C1475-1484.
Benink, H. A. and Bement, W. M. (2005) J. Cell Biol. 168: 429-439.
Fleming, Y. M. et al. (2004) J. Cell Sci. 117: 2377-2388.
Mammoto, A. et al. (2004) J. Biol. Chem. 279: 26323-26330.
Pellegrino, M. et al. (2004) J. Biol. Chem. 279: 6526-6533.
Simpson, K. J. et al. (2004) Cancer Res. 64: 8694-8701.
Burakov, A. et al. (2003) J. Cell Biol. 162: 963-969.
Zhang, X. F. et al. (2003) Neuron 40: 931-944.
Valderrama, F. et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1560-1565.
Question 1: Does Cytoskeleton sell lysis buffer for activation assays?
Answer 1: Unfortunately Cytoskeleton does not sell lysis buffer for the activation assays. The lysis buffers differ by kit which is why we include the uniquely-formulated and tested buffer with each activation assay.
Question 2: Which is the most stable/brightest Acti-stain conjugate?
Answer 2: The brightest and most stable of the Acti-stains is Acti-stain 488 (green fluorescence; Cat. # PHDG1). Please see the table below for additional information on all of our Acti-stains.