Tubulin protein: Agaricus bisporus
Product Uses Include
- Screening for fungal tubulin specific drugs
- Examining interactions of proteins with fungal tubulin
Tubulin protein has been isolated from the mushroom, Agaricus bisporus, and purified by anion exchange chromatography. Tubulin consists of one α and one β subunit. Each subunit is 55 kDa in size, as demonstrated by SDS-PAGE analysis. Typically, the molar equivalent of tubulin is defined as the heterodimer, which has a molecular weight of 110 kDa. Tubulin is supplied as a white lyophilized powder in the heterodimeric form.
Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein from 4-20% SDS-PAGE. F001 is determined to be >90% fungal tubulin
The biological activity of F001 is assessed by a tubulin polymerization assay. The polymerization of tubulin into microtubules is detected by measuring the optical density at 340 nm (see Figure 2). Under the experimental conditions defined below, a 100 µl solution of F001 at 1.0 mg/ml in a 1/2 area 96-well plate results in a change in optical density of 0.28-0.30 within 1 h at 30°C.
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