Signal-Seeker™: Validation Data

 

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Acetylation Validation Data:

Ishihara A. et al. 2019. Seasonal acclimatization and thermal acclimation induce global histone epigenetic changes in liver of bullfrog (Lithobates catesbeianus) tadpole. Comp Biochem Physiol A Mol Integr Physiol. doi: 10.1016/j.cbpa.2018.12.014. -- Anti-Acetyl Lysine Monoclonal: (AAC01-S) --Application:

Horita H. et al. 2018. Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Proteomes. doi: 10.3390/proteomes6020024. -- Signal-Seeker™ kit BK163 (Ac) --Application: IP; Anti-Acetyl Lysine Monoclonal: (AAC02) --Application: IF

Yang Y. et al. 2018. Identification of Acetylated Proteins in Borrelia burgdorferi. Methods Mol Biol. 2018;1690:177-182. doi: 10.1007/978-1-4939-7383-5_14. -- Anti-Acetyl Lysine Monoclonal: (AAC01) --Application: IP

Horita H. et al. 2017. A simple toolset to identify endogenous post-translational modifications for a target protein:a snapshot of the EGFR signaling pathway. Bioscience Reports. 37 BSR20170919 DOI: 10.1042/BSR20170919. -- Signal-Seeker™ kit BK163 (Ac) --Application: IP

LaBarge S.A. et al. 2016. p300 is not required for metabolic adaptation to endurance exercise training. FASEB J. Doi: 10.1096/fj.15-281741.-- Anti-Acetyl Lysine Monoclonal: (AAC01) --Application: IF

ASCB 2017 Poster: Tracking changes in mitochondrial acetylation by immunofluorescence provides new insight into HDACs class I/II and class III crosstalk -- Anti-Acetyl-Lysine Monoclonal: (AAC02), Signal-Seeker™ kit BK163 (Ac)

ASCB 2017 Poster: Paclitaxel induces post-translational modifications of RhoGDI alpha: a potential mechanism to regulate RhoA activity -- Signal-Seeker™ kits: BK 162 (SUMO 2/3), and BK163 (Ac)

ASCB 2016 Poster: A Method to Examine Temporal Regulation of Endogenous PTMs on Any Protein of Interest: A Snapshot of the EGFR Signaling Pathway -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)

Acetyl Lysine White Paper: Utilization of newly developed mouse monoclonal anti-acetyl lysine antibodies in western blot and immunoprecipitation applications

Anti-Acetyl Lysine Mitochondrial Protein Immunofluorescence White Paper

SUMOylation 2/3 Validation Data:

Dubuisson L. et al. 2018. Stability of HTLV-2 antisense protein is controlled by PML nuclear bodies in a SUMO-dependent manner. Oncogene. doi: 10.1038/s41388-018-0163-x. -- Signal-Seeker™ kit BK162 (SUMO 2/3) --Application: IP

Kaci A. et al. 2018. The E3 SUMO ligase PIASγ is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1α. Sci Rep. doi: 10.1038/s41598-018-29448-w. -- Signal-Seeker™ kit BK162 (SUMO 2/3) --Application: IP

Horita H. et al. 2018. Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Proteomes. doi: 10.3390/proteomes6020024. -- Signal-Seeker™ kit BK162 (SUMO 2/3) --Application: IP

Horita H. et al. 2017. A simple toolset to identify endogenous post-translational modifications for a target protein:a snapshot of the EGFR signaling pathway. Bioscience Reports. 37 BSR20170919 DOI: 10.1042/BSR20170919. -- Signal-Seeker™ kit BK162 (SUMO 2/3) --Application: IP

ASCB 2017 Poster: Paclitaxel induces post-translational modifications of RhoGDI alpha: a potential mechanism to regulate RhoA activity -- Signal-Seeker™ kits: BK 162 (SUMO 2/3), and BK163 (Ac)

ASCB 2016 Poster: A Method to Examine Temporal Regulation of Endogenous PTMs on Any Protein of Interest: A Snapshot of the EGFR Signaling Pathway -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)

SUMOylation 2/3 White Paper: Validation of SUMO-2/3 Antibodies (12F3 and 11G2) in Western Blot, Immunoprecipitation and Immunofluorescence Applications  

SUMO 2/3 White Paper: Tips for utilizing SUMO 2/3 Detection Reagents and Kits to identify endogenous SUMOylation 2/3 modified target proteins in cells and tissue

Tyrosine Phosphorylation Validation Data:

Seki T. et al. 2018. Ablation of endothelial VEGFR1 improves metabolic dysfunction by inducing adipose tissue browning. J Exp Med. doi: 10.1084/jem.20171012. -- Anti-Phosphotyrosine Monoclonal: (APY03) --Application: IP

Horita H. et al. 2018. Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Proteomes. doi: 10.3390/proteomes6020024. -- Signal-Seeker™ kit BK160 (pY) --Application: IP

Kline A. et al. 2018. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Dev Biol. pii: S0012-1606(18)30067-8. doi: 10.1016/j.ydbio.2018.05.006. -- Anti-Phosphotyrosine Monoclonal: (APY03)

Horita H. et al. 2017. A simple toolset to identify endogenous post-translational modifications for a target protein:a snapshot of the EGFR signaling pathway. Bioscience Reports. 37 BSR20170919 DOI: 10.1042/BSR20170919. -- Signal-Seeker™ kit BK160 (pY) --Application: IP

Yusuf M.Z. et al. 2017. Prostacyclin reverses platelet stress fibre formation causing platelet aggregate instability. Sci Rep. Doi: 10.1038/s41598-017-05817-9.-- Anti-Phosphotyrosine Monoclonal: (APY03) --Application: IF

Horita H. et al. 2017. Identifying regulatory post-translational modifications of PD-L1: a focus on mono-ubiquitination. Neoplasia. DOI: 10.1016/j.neo.2017.02.006. -- Signal-Seeker™ kit BK160 (pY) --Application: IP

Kaukonen R. et al. 2016. Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription. Nat Commun. Doi: 10.1038/ncomms12237.-- Anti-Phosphotyrosine Monoclonal: (APY03), Phosphotyrosine Affinity Beads (APY03-Beads) --Application: WB, IP

ASCB 2016 Poster: A Method to Examine Temporal Regulation of Endogenous PTMs on Any Protein of Interest: A Snapshot of the EGFR Signaling Pathway -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)

ASCB 2015 Poster: Temporal regulation of phosphotyrosine-modified Rac1 in response to epidermal growth factor stimulation -- Signal-Seeker™ kit: BK160 (pY)

Phosphotyrosine White Paper: Comparison of Phosphotyrosine Antibodies 27B10.4 and 4G10 in Immunoprecipitation, Immunofluorescence and Western Blot Applications

Ubiquitination Validation Data:

Wenzel HJ. et al. 2019. Astroglial-targeted expression of the fragile X CGG repeat premutation in mice yields RAN translation, motor deficits and possible evidence for cell-to-cell propagation of FXTAS pathology. Acta Neuropathol Commun. doi: 10.1186/s40478-019-0677-7. -- Anti-Ubiquitin Monoclonal: (AUB01) -- Application: IF

Brophy M. et al. 2019. Myeloid-Specific Deletion of Epsins 1 and 2 Reduces Atherosclerosis by Preventing LRP-1 Downregulation. Circ Res. doi: 10.1161/CIRCRESAHA.118.313028. -- Anti-Ubiquitin Monoclonal: (AUB01) -- Application: WB

Martini V. et al. 2018. Abnormal regulation of BCR signalling by c-Cbl in chronic lymphocytic leukaemia. Oncotarget. doi: 10.18632/oncotarget.25951. -- Signal-Seeker™ kit BK161(Ub) -- Application: IP

Horita H. et al. 2018. Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Proteomes. doi: 10.3390/proteomes6020024. -- Signal-Seeker™ kit BK161(Ub) -- Application: IP

Alvarez A. et al. 2017. KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence. Front Cell Infect Microbiol. 2017 Oct 24;7:450. doi: 10.3389/fcimb.2017.00450. -- Anti-Ubiquitin Monoclonal: (AUB01) --Application: WB

Horita H. et al. 2017. A simple toolset to identify endogenous post-translational modifications for a target protein:a snapshot of the EGFR signaling pathway. Bioscience Reports. 37 BSR20170919 DOI: 10.1042/BSR20170919. -- Signal-Seeker™ kit BK161 (Ub) --Application: IP

Braganza A. et al. 2017. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. Doi: 10.1074/jbc.M116.766824.-- Anti-Ubiquitin Monoclonal: (AUB01) --Application: WB

Horita H. et al. 2017. Identifying regulatory post-translational modifications of PD-L1: a focus on mono-ubiquitination. Neoplasia. DOI: 10.1016/j.neo.2017.02.006. -- Signal-Seeker™ kit BK161--Application: IP

Rahman H.N.A. et al. Selective Targeting of a Novel Epsin-VEGFR2 Interaction Promotes VEGF-Mediated Angiogenesis. Cir Res. Doi: 10.1161/CIRCRESAHA.115.307679.-- Anti-Ubiquitin Monoclonal: (AUB01) --Application: WB

Hukema R.K. et al. Reversibility of neuropathology and motor deficits in an inducible mouse model for FXTAS. Hum Mol Genet. Doi: 10.1093/hmg/ddv216.-- Anti-Ubiquitin Monoclonal: (AUB01-S) --Application: IF

ASCB 2016 Poster: A Method to Examine Temporal Regulation of Endogenous PTMs on Any Protein of Interest: A Snapshot of the EGFR Signaling Pathway -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)

Ubiquitination White Paper -- Signal-Seeker™ kits: BK161 (Ub), Ubiquitination affinity beads (UBA01-beads), Ubiquitination control beads (CUB02), HRP-Anti-Ubiquitin Monoclonal: AUB01-HRP

BlastR Lysis Filtration System:

Horita H. et al. 2018. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:10.3791/56912. -- Signal-Seeker™ kits

ASCB 2016 Poster: Complete removal of genomic DNA from cell lysate enhances the quality of protein research -- BlastR™ Filter (BLR02)

BlastR™ Buffer Comparison with conventional lysis buffers -- BlastR™ Rapid Lysate Prep kit (BLR01, BLR02)

PTM crosstalk:

Horita H. et al. 2018. Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Proteomes. doi: 10.3390/proteomes6020024. -- Signal-Seeker™ kits

Horita H. et al. 2018. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:10.3791/56912. -- Signal-Seeker™ kits

Horita H. et al. 2017. A simple toolset to identify endogenous post-translational modifications for a target protein:a snapshot of the EGFR signaling pathway. Bioscience Reports. 37 BSR20170919 DOI: 10.1042/BSR20170919. -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3), BK163 (Ac)

ASCB 2017 Poster: Paclitaxel induces post-translational modifications of RhoGDI alpha: a potential mechanism to regulate RhoA activity -- Signal-Seeker™ kits: BK 162 (SUMO 2/3), and BK163 (Ac)

ASCB 2016 Poster: A Method to Examine Temporal Regulation of Endogenous PTMs on Any Protein of Interest: A Snapshot of the EGFR Signaling Pathway -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)

cJUN White Paper -- Signal-Seeker™ kits: BK160 (pY), BK161 (Ub), BK162 (SUMO 2/3)