Product Uses Include
Adenosine triphosphate (ATP). 100 mM solution of ATP at pH 7.0 (to reduce hydrolysis) provided as a lyophilized powder. Required for G-actin stability and F-actin assembly and dynamics.
|Rodríguez-Pérez, Fernando et al.||Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion||Developmental Cell||2021||ISSN 1878-1551|
|Pack, Lindsey R. et al.||Clinical CDK4/6 inhibitors induce selective and immediate dissociation of p21 from cyclin D-CDK4 to inhibit CDK2||Nature Communications||2021||ISSN 2041-1723|
|Bashirzadeh, Yashar et al.||Encapsulated Actomyosin Patterns Drive Cell-Like Membrane Shape Changes||SSRN Electronic Journal||2021||Article Link|
|Park, Jin Suk et al.||Mechanical regulation of glycolysis via cytoskeleton architecture||Nature||2020||ISSN 1476-4687|
|Chung, Mingyu et al.||Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1||Molecular Cell||2019||ISSN 1097-4164|
|Piroli, Gerardo G. et al.||Identification of novel protein targets of dimethyl fumarate modification in neurons and astrocytes reveals actions independent of Nrf2 stabilization||Molecular and Cellular Proteomics||2019||ISSN 1535-9484|
|Martin, Jennifer A. et al.||A novel role for the actin-binding protein drebrin in regulating opiate addiction||Nature Communications||2019||ISSN 2041-1723|
|Minutti, Carlos M. et al.||A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation||Immunity||2019||ISSN 1097-4180|
|Patel, Vaibhav B. et al.||PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease||Nature Communications||2018||ISSN 2041-1723|
|Wang, J. et al.||Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 )||Acta Pharmacologica Sinica||2014||ISSN 1745-7254|
|Xiao, Xiang et al.||c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution||2013||PMID 23169788|
|Fan, Jianguo et al.||A role for γs-crystallin in the organization of actin and fiber cell maturation in the mouse lens||FEBS Journal||2012||ISSN 1742-464X|
|Butler, Suzanne C. et al.||Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms||Toxicology in Vitro||2012||ISSN 0887-2333|
|Jiwani, Shahanawaz et al.||Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerization||Biochemical and Biophysical Research Communications||2012||ISSN 0006-291X|
|Tsai, Chih Chien et al.||7-Chloro-6-piperidin-1-yl-quinoline-5,8-dione (PT-262), a novel ROCK inhibitor blocks cytoskeleton function and cell migration||Biochemical Pharmacology||2011||ISSN 0006-2952|
|Trigili, Chiara et al.||Mechanism of Action of the Cytotoxic Macrolides Amphidinolide X and J||ChemBioChem||2011||ISSN 1439-4227|
|Takamiya, Rina et al.||Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change||American journal of physiology. Cell physiology||2005||ISSN 0363--6143|
|Kumar, Narendra et al.||Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin||The Journal of biological chemistry||2004||ISSN 0021--9258|
|Fontao, Lionel et al.||The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin||Journal of cell science||2001||ISSN 0021--9533|
|Zhai, Liwei et al.||Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton||The Journal of biological chemistry||2001||ISSN 0021--9258|
|Blader, Ira J. et al.||GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro||Molecular Biology of the Cell||1999||ISSN 1059-1524|
Question 1: Can the reconstituted ATP be stored at 4°C for any length of time or does it need to be immediately snap-frozen?
Answer 1: The ATP (Cat. # BSA04) should be reconstituted to 100 mM with 1 ml of cold 100 mM Tris, pH 7.5. Set aside the volume that is needed for the day’s experiment (stable for 4 h on ice) and the remaining ATP should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen, and stored at or below -20°C. These stocks are stable for 6 months at -20°C.
Question 2: How long are solutions with ATP in them stable at 4°C if performing actin polymerization experiments?
Answer 2: Buffers containing ATP (Cat. # BSA04) will be stable for 4 h on ice. Keep on ice until ready to use. After 4 h discard the tube, do not re-freeze to use later. Prepare fresh buffers (or freshly frozen aliquot) for the next experiment.
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