GTPase Activation Assay Resources

Small G-protein activation assays measure the GTP-bound form of the protein from a cell or tissue extract.  Cytoskeleton offers activation assays for Rho and Ras GTPase family members in two formats: the traditional pull-down assay and a G-LISA assay.  The pull-downs utilize affinity beads linked to an effector protein that selectively binds the active GTPase which is then quantitated by Western blotting.  The G-LISAs use a 96-well plate that has been coated with an effector protein to selectively bind the active GTPase, followed by quantitation with enzyme-linked immunosorbent assay (ELISA) techniques... Read more