Choosing an activation assay


Step 1: Initial Considerations (Equipment, Starting Material and Cost)

The following should be considered when choosing the right activation assay for your needs.  Please continue to step 2 below  once you have reviewed these specifications.

Assay Factor


Assay Considerations



Equipment  Traditional Pull-down
  • SDS-PAGE apparatus
  • Western blot apparatus


     Consider the equipment requirements prior to deciding upon an assay format.
      • Orbital Plate shaker
      • Spectrophotometer (set to 490 nm)
      • Multichannel pipette (recommended if handling high number of samples)

        Starting material required per assay


          Traditional Pull-down
          • 300-2000 µg per assay


             If starting material is limited, e.g. primary cells, the G-LISA assay is required.
            • 5-50 µg per assay
                Traditional Pull-down
                • Combo kit (10 assays each RhoA/Rac1/Cdc42)
                • Starter kit (20 assays)
                • Regular kit (50-80 assays depending on target protein)



                  Cost per assay:  The G-LISA offers the best value per assay, particularly if you anticipate performing >50 assays.


                  Cost per kit:  The traditional pull-down assay starter kits generally provide the lowest cost per kit.  NOTE: Consider the combo kit as a cost effective choice if you require a quick assessment of the role of RhoA/Rac1/Cdc42 in your system.

                    • Available in 96 well format.  Each well can be broken off and used individually.


                      Step 2: Choose the number of assays required.


                      1 - 3

                      4 - 20

                      21 - 1000