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Activation Assays Q1

Choosing an activation assay

Step 1: Initial Considerations (Equipment, Starting Material and Cost)

The following should be considered when choosing the right activation assay for your needs. Please continue to step 2 below once you have reviewed these specifications.

Assay Factor		Assay Considerations		Recommendations
Equipment		Traditional Pull-down SDS-PAGE apparatus Western blot apparatus		Consider the equipment requirements prior to deciding upon an assay format.
Equipment		G-LISA Orbital Plate shaker Spectrophotometer (set to 490 nm) Multichannel pipette (recommended if handling high number of samples)
Starting material required per assay		Traditional Pull-down 300-2000 µg per assay		If starting material is limited, e.g. primary cells, the G-LISA assay is required.
Starting material required per assay		G-LISA 5-50 µg per assay
Cost		Traditional Pull-down Combo kit (10 assays each RhoA/Rac1/Cdc42) Starter kit (20 assays) Regular kit (50-80 assays depending on target protein)		Cost per assay: The G-LISA offers the best value per assay, particularly if you anticipate performing >50 assays. Cost per kit: The traditional pull-down assay starter kits generally provide the lowest cost per kit. NOTE: Consider the combo kit as a cost effective choice if you require a quick assessment of the role of RhoA/Rac1/Cdc42 in your system.
Cost		G-LISA Available in 96 well format. Each well can be broken off and used individually.

Step 2: Choose the number of assays required.

1 - 3

4 - 20

21 - 1000