Anti-Phosphotyrosine Clone 27B10.4. Why Switch?

Comparison of Leading Phosphotyrosine Antibodies

The results of a comparison between a newly developed phosphotyrosine antibody, clone 27B10.4 (Catalog # APY03) with the popular phosphotyrosine antibody clone 4G10 are described in this white paper.  Antibody performance was compared in immunoprecipitation, immunofluorescence, and western blot applications. In all cases the data shown are representative of at least 4 separate experiments.  We report that 27B10.4 and 4G10 have a broad similarity in phosphotyrosine-protein recognition, with both antibodies showing some sequence context differences. Clone 27B10.4 was shown to immunoprecipitate a wider diversity of phosphotyrosine-proteins than 4G10 and to detect stronger signals in low molecular weight proteins in western blot applications.  This, together with a superior performance in immunofluorescence applications, makes clone 27B10.4 an exciting new reagent for the study of phosphotyrosine regulation in health and disease.  (Read more here)